The University of Georgia

Publications

2014 Publications
2013 Publications
2012 Publications
2011 Publications
2010 Publications
2009 Publications

2014 Publications

Zhang F, Moniz HA, Walcott B, Moremen KW, Wang L, Linhardt RJ. (2014) Probing the impact of GFP tagging on Robo1-heparin interaction. Glycoconj J. in press

Frank M, Walker RC, Lanzilotta WN, Prestegard JH, Barb AW (2014) Immunoglobulin G1 Fc domain motions: implications for Fc engineering. J. Mol Biol. 426:1799-811 DOI: 10.1016/j.jmb.2014.01.011

Martin JC, Fadda E, Ito K, Woods RJ. (2014) Defining the structural origin of the substrate sequence independence of O-GlcNAcase using a combination of molecular docking and dynamics simulation. Glycobiology. 24:85-96. DOI: 10.1093/glycob/cwt094

Hadden JA, French AD, Woods RJ (2014) Effect of microfibril twisting on theoretical powder diffraction patterns of cellulose I β. Cellulose. in press.

Moremen KW, Nairn AV (2013) Mannosidase, alpha, class 2a1 (MAN2A1, Golgi α-mannosidase II). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition (Taniguchi N, ed.). Springer-Verlag, Tokyo. pp. 1313-1326.

Moremen KW, Nairn AV (2013) Mannosidase, alpha, class 1 (MAN1A1 (Golgi α-mannnosidase IA), Man1A2 (Golgi α-mannosidase IB), MAN1B1(ER α-mannosidase I), MAN1C1 (Golgi α-mannosidase IC ). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition (Taniguchi N, ed.). Springer-Verlag, Tokyo. pp. 1297-1312.

Nairn AV, Moremen KW (2014) Glucosidase, alpha neutral AB; glucosidase II subunit beta (GANAB, PRKCSH, α-glucosidase II). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition (Taniguchi N, ed.). Springer-Verlag, Tokyo. pp. 1283-1295.

Nairn AV, Moremen KW (2013) Mannosyl-oligosaccharide glucosidase (Glucosidase I, MOGS). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition (Taniguchi N, ed.). Springer-Verlag, Tokyo. pp. 1273-1282.

Prestegard, J.H., Agard, D.A., Moremen, K.W. Lavery, L.A., Morris, L.C. & Pederson, K. (2014) Sparse labeling of proteins: Structural characterization from long range constraints. J Magn Reson 241:32-40 DOI: 10.1016/j.jmr.2013.12.012

Qiu, H., Xia, W.Y., & Wang, L. (2014) Heparan sulfate modulates Slit3-induced endothelial cell migration. Methods Mol Biol, in press.

Kailemia MJ, Park M, Kaplan DA, Venot A, Boons GJ, Li L, Linhardt RJ, Amster IJ. (2014) High-field asymmetric-waveform ion mobility spectrometry and electron detachment dissociation of isobaric mixtures of glycosaminoglycans. J Am Soc Mass Spectrom. 25:258-68. DOI: 10.1007/s13361-013-0771-1

Gasimli L, Hickey AM, Yang B, Li G, Dela Rosa M, Nairn AV, Kulik MJ, Dordick JS, Moremen KW, Dalton S, Linhardt RJ. (2014) Changes in
glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages. Biochim Biophys Acta. 1840:1993-2003. DOI: 10.1016/j.bbagen.2014.01.007

Kailemia MJ, Ruhaak LR, Lebrilla CB, Amster IJ. (2014) Oligosaccharide analysis by mass spectrometry: a review of recent developments. Anal Chem. 86:196-212. DOI: 10.1021/ac403969n

Zhang B, Xiao W, Qiu H, Zhang F, Moniz HA, Jaworski A, Condac E, Gutierrez-Sanchez G, Heiss C, Clugston RD, Azadi P, Greer JJ, Bergmann C, Moremen KW, Li D, Linhardt RJ, Esko JD, Wang L. (2014) Heparan sulfate deficiency disrupts developmental angiogenesis and causes congenital diaphragmatic hernia. J Clin Invest. 124:209-21. DOI: 10.1172/JCI71090

Lu J, Auduong L, White ES, Yue X. (2014) Up-regulation of heparan sulfate 6-o-sulfation in idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol. 50:106-14. DOI: 10.1165/rcmb.2013-0204OC

2013 Publications

Barb AW, Hekmatyar SK, Glushka JN, Prestegard JH (2013) Probing alanine transaminase catalysiswith hyperpolarized 13CD3-pyruvate. J. Magn. Reson. 228: 59-65. DOI: 10.1016/j.jmr.2012.12.013

Barb AW, Wang X, Prestegard JH (2013) Refolded recombinant Siglec5 for NMR investigationof complex carbohydrate binding. Protein Expr. Purif. 88: 183-189. DOI: 10.1016/j.pep.2013.01.005

Benoit SL, Bayyareddy K, Mahawar M, Sharp JS, Maier RJ (2013) Alkyl hydroperoxide reductaserepair by Helicobacter pylori methionine sulfoxide reductase. J. Bacteriol. 195: 5396-5401.DOI: 10.1128/JB.01001-13

Chen HM, Zhen ZP, Tang W, Todd T, Chuang JY, Wang L, Pan Z, Xie J (2013) Label-free luminescentmesoporous silica nanoparticles for imaging and drug delivery. Theranostics 3: 650-657.DOI: 10.7150/thno.6668

Gao Y, Aryal RP, Ju T, Cummings RD, Gahlay G, Jarvis DL, Matta KL, Vlahakis JZ, Szarek W, Brockhausen I(2013) Acceptor specificities and selective inhibition of recombinant human Gal- and GlcNAc-transferasesthat synthesize core structures 1, 2, 3 and 4 of O-glycans. Biochem. Biophys. Acta 1830:4274-4281. DOI: 10.1016/j.bbagen.2013.04.001

Gerken T, Revoredo L, Thome JJC, Tabak LA, Vester-Christensen MB, Clausen H, Gahlay GK, Jarvis DL,Johnson RY, Moniz HA, Moremen KW (2013) The lectin domain of the polypeptide GalNAc transferase familyof glycosyltransferases (ppGalNAc T’s) acts as a switch directing glycopeptide substrateglycosylation in an N- or C-terminal direction, further controlling mucin-type O-glycosylation.J. Biol. Chem. 288: 19900-19914. DOI: 10.1074/jbc.M113.477877

Hadden JA, French AD, Woods RJ (2013) Unraveling cellulose microfibrils: A twisted tale.Biopolymers 99: 746-756. DOI: 10.1002/bip.22279

Heimburg-Molinaro J, Priest JW, Live D, Boons GJ, Song X, Cummings RD, Mead JR (2013) Microarrayanalysis of the human antibody response to synthetic Cryptosporidium glycopeptides.Int. J. Parasitol. 43: 901-907. DOI: 10.1016/j.ijpara.2013.05.012

Huang R, Liu J, Sharp JS (2013) An approach for separation and complete structural sequencingof heparin/heparan sulfate-like oligosaccharides. Anal. Chem. 85: 5787-5795.DOI: 10.1021/ac400439a

Kailemia MJ, Li L, Xu Y, Liu J, Linhardt RJ, Amster IJ (2013) Structurally informative tandem massspectrometry of highly sulfated natural and chemoenzymatically synthesized heparin and heparansulfate glycosaminoglycans. Mol. Cell. Proteomics 12: 979-990. DOI: 10.1074/mcp.M112.026880

Kraushaar DC, Dalton S, Wang L (2013) Heparan sulfate: A key regulator of embryonic stem cell fate.Biol. Chem. 394: 741-751. DOI: 10.1515/hsz-2012-0353

Kuhns LG, Mahawar M, Sharp JS, Maier RJ (2013) Role of Helicobacter pylori methionine sulfoxidereductase in urease maturation. Biochem. J. 450: 141-148. DOI: 10.1042/BJ20121434

Li X, Li Z, Xie B, Sharp JS (2013) Improved identification and relative quantification of sites ofpeptide and protein oxidation for hydroxyl radical footprinting. J. Am. Soc. Mass Spectrom.24: 1767-1776. DOI: 10.1007/s13361-013-0719-5

Live D, Wells L, Boons GJ (2013) Dissecting the molecular basis for the role of O-mannosylationpathway in disease: α-Dystroglycan and forms of muscular dystrophy. ChemBioChem14: 2392-2402. DOI: 10.1002/cbic.201300417

Mbua NE, Flanagan-Steet H, Johnson S, Wolfert MA, Boons GJ, Steet R (2013) Abnormal accumulationand recycling of sialylated glycoproteins visualized in Niemann-Pick type C cells using the chemicalreporter strategy. Proc. Natl. Acad. Sci. USA 110: 10207-10212. DOI: 10.1073/pnas.1221105110

Mbua NE, Li X, Flanagan-Steet HR, Meng L, Aoki K, Moremen KW, Wolfert MA, Steet R, Boons GJ (2013)Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I.Angew. Chem., Int. Ed. 52: 13012-13015. DOI: 10.1002/anie.201307095

Meng L, Forouhar F, Thieker D, Gao Z, Ramiah A, Moniz H, Seetharaman J, Milaninia S, Su M, Bridger R,Veillon L, Azadi P, Kornhaber G, Wells L, Montelione GT, Woods RJ, Tong L, Moremen KW (2013) Enzymaticbasis for N-glycan sialylation: Structure of rat α2,6-sialyltransferase (ST6GAL1)reveals conserved and unique features for glycan sialylation. J. Biol. Chem. 288: 34680-34698.DOI: 10.1074/jbc.M113.519041

Prestegard JH, Sahu SC, Nkari WK, Morris LC, Live D, Gruta C (2013) Chemical shift prediction fordenatured proteins. J. Biomol. NMR 55: 201-209. DOI: 10.1007/s10858-012-9702-x

Qiu H, Jiang JL, Liu M, Huang X, Ding SJ, Wang L (2013) Quantitative phosphoproteomics analysis revealsbroad regulatory role of heparan sulfate on endothelial signaling. Mol. Cell. Proteomics12: 2160-2173. DOI: 10.1074/mcp.M112.026609

Tessier MB, Grant OC, Heimburg-Molinaro J, Smith D, Jadey S, Gulick AM, Glushka J, Deutscher SL, Rittenhouse-Olson K, Woods RJ (2013) Computational screening of the human TF-glycome providesa structural definition for the specificity of anti-tumor antibody JAA-F11. PLoS One8: e54874. DOI: 10.1371/journal.pone.0054874

Wang X, Sharp JS, Handel TM, Prestegard JH (2013) Chemokine oligomerization in cell signalingand migration. Prog. Mol. Biol. Transl. Sci. 117: 531-578. DOI: 10.1016/B978-0-12-386931-9.00020-9

Wang Z, Chinoy Z, Ambre S, Peng W, McBride R, de Vries RP, Glushka J, Paulson JC, Boons GJ (2013)A general strategy for the chemoenzymatic synthesis of asymmetrically branched N-glycans.Science 341: 379-383. DOI: 10.1126/science.1236231

Zhang FM, Moniz HA, Walcott B, Moremen KW, Linhardt RJ, Wang L (2013) Characterization of theinteraction between Robo1 and heparin and other glycosaminoglycans. Biochimie95: 2345-2353. DOI: 10.1016/j.biochi.2013.08.018

Lin CH, Jarvis DL.(2013) Utility of temporally distinct baculovirus promoters for constitutive and baculovirus-inducible transgene expression in transformed insect cells. J Biotechnol. 165:11-7. DOI: 10.1016/j.jbiotec.2013.02.007

Langeslay DJ, Beecher CN, Naggi A, Guerrini M, Torri G, Larive CK. (2013) Characterizing the microstructure of heparin and heparan sulfate using N-sulfoglucosamine 1H and 15N NMR chemical shift analysis. Anal Chem. 85:1247-55. DOI: 10.1021/ac3032788

Zhou B, Honor LB, He H, Ma Q, Oh JH, Butterfield C, Lin RZ, Melero- Martin JM, Dolmatova E, Duffy HS, Gise Av, Zhou P, Hu YW, Wang G, Zhang B, Wang L, Hall JL, Moses MA, McGowan FX, Pu WT. (2011) Adult mouse epicardium modulates myocardial injury by secreting paracrine factors. J Clin Invest. 121:1894-904. DOI: 10.1172/JCI45529

2012 Publications

Barb AW, Ho TG, Flanagan-Steet H, Prestegard JH (2013) Lanthanide binding and IgG affinity construct: Potential applications in solution NMR, MRI, and luminescence microscopy. Protein Sci. 21: 1456-1466.DOI: 10.1002/pro.2133

Barb AW, Meng L, Gao Z, Johnson RW, Moremen KW, Prestegard JH (2012) NMR characterization of immunoglobulinG Fc glycan motion on enzymatic sialylation. Biochemistry 51: 4618-4626. DOI: 10.1021/bi300319q

Bogert A, Heimburg-Molinaro J, Song X, Lasanajak Y, Ju T, Liu M, Thompson P, Ragupathi G, Barany G,Smith DF, Cummings RD, Live D (2012) Deciphering structural elements of mucin glycoprotein recognition.ACS Chem. Biol. 7: 1031-1039. DOI: 10.1021/cb300076s

Condac E, Strachan H, Gutierrez-Sanchez G, Brainard B, Giese C, Heiss C, Johnson D, Azadi P, Bergmann C,Orlando R, Esmon CT, Harenberg J, Moremen K, Wang L (2012) The C-terminal fragment of axon guidancemolecule Slit3 binds heparin and neutralizes heparin’s anticoagulant activity. glycobiology22: 1183-1192.DOI: 10.1093/glycob/cws087

Davies LRL, Pearce OMT, Tessier MB, Assar S, Smutova V, Pajunen M, Sumida M, Sato C, Kitajima K, Finne J,Gagneux P, Pshezhetsky A, Woods RJ, Varki A (2012) Metabolism of vertebrate amino sugars with N-glycolylgroups: Resistance of α 2-8-linked N-glycolylneuraminic acid to enzymatic cleavage. J. Biol. Chem. 287: 28917-28931. DOI: 10.1074/jbc.M112.365056

Friscourt F, Fahrni CJ, Boons GJ (2012) A fluorogenic probe for the catalyst-free detection ofazide-tagged molecules. J. Am. Chem. Soc. 134: 18809-18815. DOI: 10.1021/ja309000s

Friscourt F, Ledin PA, Mbua NE, Flanagan-Steet HR, Wolfert MA, Steet R, Boons GJ (2012) Polardibenzocyclooctynes for selective labeling of extracellular glycoconjugates of living cells.J. Am. Chem. Soc. 134: 5381-5389. DOI: 10.1021/ja3002666

Guo C, Wang B, Wang L, Xu B (2012) Structural basis of single molecular heparin-FX06 interactionrevealed by SPM measurements and molecular simulations. Chem. Commun. 48: 12222-12224.DOI: 10.1039/c2cc36546d

Johnson MA, Cartmell J, Weisser NE, Woods RJ, Bundle DR (2012) Molecular recognition of Candidaalbicans (1→2)-β-mannan oligosaccharides by a protective monoclonal antibody reveals theimmunodominance of internal saccharide residues. J. Biol. Chem. 287: 18078-18090.DOI: 10.1074/jbc.M112.355578

Kraushaar DC, Rai S, Condac E, Nairn A, Zhang SY, Yamaguchi Y, Moremen K, Dalton S, Wang L (2012)Heparan sulfate facilitates FGF and BMP signaling to drive mesoderm differentiation of mouse embryonicstem cells. J. Biol. Chem. 287: 22691-22700. DOI: 10.1074/jbc.M112.368241

Lamanna WC, Lawrence R, Sarrazin S, Lameda-Diaz C, Gordts PL, Moremen KW, Esko JD (2012) A genetic modelof substrate reduction therapy for mucopolysaccharidosis. J. Biol. Chem. 287: 36283-36290.DOI: 10.1074/jbc.M112.403360

Lawrence R, Brown JR, Al-Mafraji K, Lamanna WC, Beitel JR, Boons GJ, Esko JD, Crawford BE (2012)Disease-specific non-reducing end carbohydrate biomarkers for mucopolysaccharidoses. Nature Chem. Biol.8: 197-204. DOI: 10.1038/nchembio.766

Leach FE III, Arungundram S, Al-Mafraji K, Venot A, Boons GJ, Amster IJ (2012) Electron detachment dissociationof synthetic heparin sulfate glycosaminoglycan tetrasaccharides varying in degree of sulfation andhexuronic acid stereochemistry. Int. J. Mass Spectrom. 330-332: 152-159. DOI: 10.1016/j.ijms.2012.07.002

Moremen KW, Tiemeyer M, Nairn AV (2012) Vertebrate protein glycosylation: Diversity, synthesis andfunction. Nature Rev. Mol. Cell. Biol. 13: 448-462. DOI: 10.1038/nrm3383

Pomin VH, Park Y, Huang R, Heiss C, Sharp JH, Azadi P, Prestegard JH (2012) Exploiting enzyme specificities indigestions of chondroitin sulfates A and C: Production of well-defined hexasaccharides. Glycobiology22: 826-838. DOI: 10.1093/glycob/cws055

Satelle BM, Bose-Basu B, Tessier M, Woods RJ, Serianni AS, Almond A (2012) Dependence of pyranose ringpuckering on anomeric configuration: Methyl idopyranosides. J. Phys. Chem. B 116: 6380-6386.DOI: 10.1021/jp303183y

Tran DT, Lim JM, Liu M, Stalnaker SH, Wells L, Ten Hagen KG, Live D (2012) Glycosylation ofα-dystroglycan: O-mannosylation influences the subsequent addition of GalNAc by UDP-GalNAc polypeptideN-acetylgalactosaminyltransferases. J. Biol. Chem. 287: 20967-20974. DOI: 10.1074/jbc.M112.370387

Watson C, Sharp JS (2012) Conformational analysis of therapeutic proteins by hydroxyl radicalprotein footprinting. AAPS J. 14: 206-217. DOI: 10.1208/s12248-012-9336-7

Zhang S, Condac E, Qiu H, Jiang J, Gutierrez-Sanchez G, Bergmann C, Handel T, Wang L (2012) Heparin-inducedleukocytosis requires 6-O-sulfation and is caused by blockade of selectin- and CXCL12 protein-mediatedleukocyte trafficking in mice. J. Biol. Chem. 287: 5542-5553. DOI: 10.1074/jbc.M111.314716

Wang X. (2012) Solution structure of decorin-binding protein A from Borrelia burgdorferi. Biochemistry. 51:8353-62. DOI: 10.1021/bi3007093

Nguyen TK, Arungundram S, Tran VM, Raman K, Al-Mafraji K, Venot A, Boons GJ, Kuberan B. (2012) A synthetic heparan sulfate oligosaccharide library reveals the novel enzymatic action of D-glucosaminyl 3-Osulfotransferase-3a. Mol Biosyst. 8:609-14. DOI: 10.1039/c1mb05221g

2011 Publications

Barb AW, Freedberg DI, Battistel MD, Prestegard JH (2011) NMR detection and characterization ofsialylated glycoproteins and cell surface polysaccharides. J. Biomol. NMR 51: 163-171.DOI: 10.1007/s10858-011-9550-0

Barb AW, Hekmatyar SK, Glushka JN, Prestegard JH (2011) Exchange facilitate indirect detectionof hyperpolarized 15ND2-amido-glutamine. J. Magn. Reson. 212: 304-310. DOI: 10.1016/j.jmr.2011.07.008

Barb AW, Prestegard JH (2011) NMR analysis demonstrates immunoglobulin G N-glycans are accessibleand dynamic. Nature Chem. Biol. 7: 147-153. DOI: 10.1038/nchembio.511

Barb A, Prestegard JH. (2011) Accessibility and Dynamics of Immunoglobulin G N-glycans.Nature Chemical Biology 7(3):147-153. DOI: 10.1038/nchembio.511

DeMarco ML, Woods RJ (2011) From agonist to antagonist: Structure and dynamics of innate immuneglycoprotein MD-2 upon recognition of variably acylated bacterial endotoxins. Mol. Immunol.49: 124-133. DOI: 10.1016/j.molimm.2011.08.003

Heiss C, Wang Z, Azadi P (2011). Sodium hydroxide permethylation of heparin disaccharides.Rapid Commun. Mass Spectrom. 25:774-778. DOI: 10.1002/rcm.4930

Hoenig M, Jordan ET, Glushka J, Kley S, Patil A, Waldron M, Prestegard JH, Ferguson DC, Wu S,Olson DE (2011) Effect of macronutrients, age, and obesity on 6- and 24-h postprandial glucosemetabolism in cats. Am. J. Physiol. Regul. Integr. Comp. Physiol. 301: R1798-1087.DOI: 10.1152/ajpregu.00342.2011

Huang, R., Pomin, V. H. & Sharp, J. S. (2011). LC-MSn analysis of isomeric chondroitin sulfateoligosaccharides using a chemical derivatization strategy. J. Am. Soc. Mass Spectrom. 22:1577-1587.DOI: 10.1007/s13361-011-0174-0

Ju R, Tessier M, Olliff L, Woods RJ, Summers A, Geng Y (2011) Supramolecular dendrimer capsulesby cooperative binding. ChemComm. 47(1):268-70. DOI: 10.1039/c0cc01983f

Laremore TN, Leach III FE, Amster IJ, Linhardt RJ (2011) Electrospray ionization fourier transformmass spectrometric analysis of intact bikunin glycosaminoglycan from normal human plasma. Int. J. MassSpectrom. 305(2-3):109-115. DOI: 10.1016/j.ijms.2010.09.020

Leach FE 3rd, Wolff JJ, Xiao Z, Ly M, Laremore TN, Arungundram S, Al-Mafraji K, Venot A,Boons GJ, Linhardt RJ, Amster IJ (2011). Negative electron transfer dissociation Fourier transformmass spectrometry of glycosaminoglycan carbohydrates. Eur. J. Mass Spectrom. 17:167-176. DOI: 10.1255/ejms.1120

Mahawar M, Tran V, Sharp JS, Maier RJ (2011). Synergistic roles of Helicobacter pylorimethionine sulfoxide reductase and GroEL in repairing oxidant-damaged catalase. J. Biol. Chem.286:19159-19169. DOI: 10.1074/jbc.M111.223677

Mo KF, Fang T, Stalnaker SH, Kirby PS, Liu M, Wells L, Pierce M, Live DH, Boons GJ (2011). Synthetic,structural, and biosynthetic studies of an unusual phospho-glycopeptide derived from α-dystroglycan.J. Am. Chem. Soc. 133:14418-14430. DOI: 10.1021/ja205473q

Oh HB, Leach FE 3rd, Arungundram S, Al-Mafraji K, Venot A, Boons GJ, Amster IJ (2011). Multivariateanalysis of electron detachment dissociation and infrared multiphoton dissociation mass spectra ofheparan sulfate tetrasaccharides differing only in hexuronic acid stereochemistry. J. Am. Soc. MassSpectrom. 22:582-590. DOI: 10.1007/s13361-010-0047-y

Wang X, Lee HW, Liu Y, Prestegard JH (2011). Structural NMR of protein oligomers using hybrid methods.J. Struct. Biol. 173:515-529. DOI: 10.1016/j.jsb.2010.11.005

Wang X, Watson C, Sharp JS, Handel TM, Prestegard JH (2011) Oligomeric Structure of the ChemokineCCL5/RANTES from NMR, MS and SAXS Data. Structure 19(8):1138-1148. DOI: 10.1038/nsmb.1853

2010 Publications

Bern M, Saladino J, Sharp JS (2010) Conversion of methionine into homcysteic acid in heavily oxidized proteomics samples. Rapid Commun. Mass Spectrom. 24: 768-772. DOI: 10.1002/rcm.4447

DeMarco ML, Woods RJ, Prestegard JH, Tian F (2010) Presentation of membrane-anchored glycosphingolipidsdetermined from molecular dynamics simulations and NMR paramagnetic relaxation rate enhancement. J. Am. Chem. Soc. 132: 1334-1338. DOI: 10.1021/ja907518x

Fadda E, Woods RJ (2010) Molecular simulations of carbohydrates and protein-carbohydrate interactions:Motivation, issues and prospects. Drug Disc. Today 15: 596-609. DOI: 10.1016/j.drudis.2010.06.001

Fuster MM, Wang L (2010) Endothelial heparan sulfate in angiogenesis. Prog. Mol. Biol. Transl. Sci.93C: 179-212. *co-first author DOI: 10.1016/S1877-1173(10)93009-3

Gu X, Glushka J, Lee SG, Bar-Peled M (2010) Biosynthesis of a new UDP-sugar, UDP-2-acetamido-2-deoxyxylose,UDP-2-acetamido-2-deoxyxylose, in the human pathogen Bacillus cereus subspecies cytotoxis NVH 391-98.J. Biol. Chem. 285: 24825-24833. DOI: 10.1074/jbc.M110.125872

Gu X, Glushka J, Yin Y, Xu Y, Denny T, Smith J, Jiang Y, Bar-Peled M (2010) Identification of abifunctional UDP-4-keto-pentose/UDP-xylose synthase in the plant pathogenic bacterium Ralstonia solanacearum strain GMI1000, a distinct member of the 4,6-dehydratase and decarboxylase family. J. Biol. Chem. 285: 9030-9040. DOI: 10.1074/jbc.M109.066803

Jiang Y, McKinnon T, Varatharajan J, Glushka J, Prestegard JH, Sornborger AT, Schuttler H-B,Bar-Peled M (2010) Time-resolved NMR: Extracting the topology of complex enzyme networks. Biophysical J. 99(7):2318-2326. DOI: 10.1016/j.bpj.2010.08.014

Ning X, Temming RP, Dommerholt J, Guo J, Ania DB, Debets MF, Wolfert MA, Boons GJ, van Delft FL (2010) Protein modification by strain-promoted alkyne-nitrone cycloaddition. Angew. Chem. Int. Ed.49: 3065-3068. DOI: 10.1002/anie.201000408

Pomin VH, Sharp JS, Li X, Wang L, Prestegard JH (2010) Characterization of glycosaminoglycans by 15N NMR spectroscopy and in vivo isotopic labeling. Anal. Chem. 82: 4078-4088. DOI: 10.1021/ac1001383

Raman S, Lange OF, Rossi P, Tyka M, Wang X, Aramini J, Liu G, Ramelot TA, Eletsky A, Szyperski T, Kennedy MA, Prestegard J, Montelione GT, Baker D (2010) NMR structure determination for larger proteinsusing backbone-only data. Science 327: 1014-1018. DOI: 10.1126/science.1183649
Shapira B, Prestegard JH (2010) Electron-nuclear interactions as probes of domain motion in proteins. J. Chem. Phys. 132: 115102-115113. DOI: 10.1063/1.3328644

Teo CF, Ingale S, Wolfert MA, Elsayed GA, Nöt LG, Chatham JC, Wells L, Boons GJ (2010) Glycopeptide-specificmonoclonal antibodies suggest new roles for O-GlcNAc. Nature Chem. Biol. 6: 338-343. DOI: 10.1038/nchembio.338

Wijelath E, Namekata M, Murray J, Furuyashiki M, Zhang S, Coan D, Wakao M, Harris RB, Suda Y, Wang L, Sobel M (2010) Multiple mechanisms for exogenous heparin modulation of vascular endothelial growth factoractivity. J. Cell Biochem. 111(2): 461-468. DOI: 10.1002/jcb.22727

Wolff JJ, Leach FE 3rd, Laremore TN, Kaplan DA, Easterling ML, Linhardt RJ, Amster IJ (2010) Negative electron transfer dissociation of glycosaminoglycans. Anal. Chem. 82: 3460-3466.DOI: 10.1021/ac100554a

Woods RJ, Tessier M (2010) Computational glycoscience: Characterizing the spatial and temporal properties of glycans and glycan-protein complexes. Curr. Opin. Struct. Biol. 20: 1-9. DOI: 10.1016/j.sbi.2010.07.005

Kelly U, Yu L, Kumar P, Ding JD, Jiang H, Hageman GS, Arshavsky VY, Frank MM, Hauser MA, Rickman CB. (2010) Heparan sulfate, including that in Bruch’s membrane, inhibits the complement alternative pathway: implications for age-related macular degeneration. J Immunol. 185:5486-94. DOI: 10.4049/jimmunol.0903596

Kumar A, Hou X, Lee C, Li Y, Maminishkis A, Tang Z, Zhang F, Langer HF, Arjunan P, Dong L, Wu Z, Zhu LY, Wang L, Min W, Colosi P, Chavakis T, Li X. (2010) Platelet-derived growth factor-DD targeting arrests pathological angiogenesis by modulating glycogen synthase kinase-3beta phosphorylation. J Biol Chem. 85:15500-10. 10.1074/jbc.M110.113787

Shapira B, Prestegard JH. (2010) Electron-nuclear interactions as probes of domain motion in proteins. J Chem Phys. 132:115102. DOI: 10.1063/1.3328644

Zhu Y, Suits MD, Thompson AJ, Chavan S, Dinev Z, Dumon C, Smith N, Moremen KW, Xiang Y, Siriwardena A, Williams SJ, Gilbert HJ, Davies GJ. (2010) Mechanistic insights into a Ca2+-dependent family of alpha-mannosidases in a human gut symbiont. Nat Chem Biol. 6:125-32. DOI: 10.1038/nchembio.278

Stanford KI, Wang L, Castagnola J, Song D, Bishop JR, Brown JR, Lawrence R, Bai X, Habuchi H, Tanaka M, Cardoso WV, Kimata K, Esko JD. (2010) Heparan sulfate 2-O-sulfotransferase is required for triglyceride-rich lipoprotein clearance. J Biol Chem. 285:286-94. DOI: 10.1074/jbc.M109.063701

2009 Publications

Arungundram S, Al-Mafraji K, Asong J, Leach FE III, Amster IJ, Venot A, Turnbull JE, Boons GJ (2009) Modular synthesis of heparan sulfate oligosaccharides for structure-activity relationship studies.J. Am. Chem. Soc. 131: 17394-17405. DOI: 10.1021/ja907358k

Barb AW, Brady EK, Prestegard JH (2009) Branch specific sialylation of IgG-Fc glycans by ST6Gal-I.Biochemistry 48: 9705-9707. DOI: 10.1021/bi901430h

Chen G, Ning X, Park B, Boons GJ, Xu B (2009) Simple, clickable protocol for atomic force microscopytip modification and its application for trace ricin detection by recognition imaging. Langmuir 25: 2860-2864. DOI: 10.1021/la803523b

DeMarco ML, Woods RJ (2009) Atomic-resolution conformational analysis of the GM3 ganglioside in alipid biolayer, and its implications for ganglioside-protein recognition at membrane surfaces.Glycobiology 19: 344-355. DOI: 10.1093/glycob/cwn137

Gau BC, Sharp JS, Rempel DL, Gross ML (2009) Fast photochemical oxidation of proteins footprintsfaster than protein unfolding. Anal. Chem. 81: 6563-6571. DOI: 10.1021/ac901054w

Guyett P, Glushka J, Gu X, Bar-Peled M (2009) Real-time NMR monitoring of intermediates and labile products of the bifunctional enzyme UDP-apiose/UDP-xylose synthase. Carbohydr. Res.344: 1072-1078. DOI: 10.1016/j.carres.2009.03.026

Kley S, Hoenig M, Glushka J, Jin ES, Burgess SC, Waldron M, Jordan ET, Prestegard JH, Ferguson DC,Wu S, Olson DE (2009) The impact of obesity, sex and diet on hepatic glucose production in cats.Am. J. Physiol. Regul. Integr. Comp. Physiol. 296: R936-943. DOI: 10.1152/ajpregu.90771.2008

Kuntz DA, Zhong W, Guo J, Rose DR, Boons GJ (2009) The molecular basis of inhibition of Golgi alpha-mannosidase II by mannostatin A. ChemBioChem. 10: 268-277. DOI: 10.1002/cbic.200800538

Liu M, Alvarez-Manilla G, Brothers F, Live D (2009) Fluorescently labeled glycopeptides for assay of POMGnT1 activity and muscle-eye-brain disease. In: Proceedings of the 21st American Peptide Symposium(M. Lebl, ed.), pp. 4-42. Prompt Scientific Publishing, San Diego, CA.

Liu S, Meng L, Moremen KW, Prestegard JH (2009) NMR structural characterization of substrates bound to the α-2, 6-sialyltransferase, ST6Gal-I. Biochemistry 48: 11211-11219. DOI: 10.1021/bi9015154

Live D, Borgert A, Barany G, Liu M (2009) Structural studies of mucin glycoprotein motifs. In: Proceedings of the 21st American Peptide Symposium (M. Lebl, ed.), pp. 353-355. Prompt Scientific Publishing, San Diego, CA.

Orlando R, Lim J, Atwood JA III, Angel PM, Fang G, Alvarez-Manilla G, Moremen KW, York WS, Tiemeyer M, Pierce M, Dalton S, Wells L (2009) IDAWG: Metabolic incorporation of stable isotope labels for quantitative glycomics of cultured cells. J. Proteome Res. 8: 3816-3823.DOI: 10.1021/pr8010028

Saladino J, Liu M, Live D, Sharp JS (2009) Aliphatic peptidyl hydroperoxides as a source of secondary oxidation in hydrosyl radical protein footprinting. J. Am. Soc. Mass Spectrom.20: 1123-1126. DOI: 10.1016/j.jasms.2009.02.004

Termine DJ, Moremen KW, Sifers RN (2009) The mammalian UPR boosts glycoprotein ERAD by suppressingthe down-regulation of ER mannosidase I. J. Cell Sci. 122: 976-984. DOI: 10.1242/jcs.037291

Watson C, Janik I, Zhuang T, Charvatova O, Woods RJ, Sharp J (2009) Pulsed electron beam water radiolysis for sub-microsecond hydroxyl radical protein footprinting. Anal. Chem.81: 2496-2505. DOI: 10.1021/ac802252y

Wolff JJ, Laremore TN, Leach FE, Linhardt RJ, Amster IJ (2009) Electron capture dissociation, electron detachment dissociation, and infrared multiphoton dissociation of sucrose octasulfate.Eur. J. Mass Spectrom. 15: 275-281. DOI: 10.1255/ejms.951

Zhang B, Dietrich UM, Geng JG, Bicknell R, Esko JD, Wang L (2009) Repulsive axon guidancemolecule Slit3 is a novel angiogenic factor. Blood 114: 4300-4309. DOI: 10.1182/blood-2008-12-193326