Author: jennbryant

Although hundreds of heparan sulfate binding proteins have been identified, and implicated in a myriad of physiological and pathological processes, very little information is known about ligand requirements for binding and mediating biological activities by these proteins. This difficulty results from a lack of technology for establishing structure-activity-relationships, which in turn is due to the …

Link modules found in a number of cell surface proteins play important roles in stabilizing the extracellular matrix (ECM) by interacting with glycosaminoglycans (GAGs). However the specificity and geometry of these interactions is poorly defined. Driven by a collaborative project with the Tony Day lab of the University of Manchester, Younghee Park in the Prestegard …

Co-receptor function of heparan sulfate in Slit3-Robo4 signaling: Heparan sulfate, carried by the heparan sulfate proteoglycans, such as syndecan, binds Slit3 on wildtype endothelial cell surface to form the heparan sulfate-Slit3-Robo4 ternary complexes facilitating Slit3-Robo4 signaling. Genetic ablation of N-deacetylase-N-sulfotransferase-1 (Ndst1), an dual function enzyme that initiates modification reactions during heparan sulfate biosynthesis, reduces the …

A platform for recombinant expression of mammalian glycoproteins has been developed in the Moremen lab for biochemical and structural studies on glycosylation enzymes and glycan binding proteins. Successful production of mammalian glycosylation enzymes in the Moremen lab led to the expanded development of a large-scale project for the expression of all mammalian glycosylation enzymes through …

A team of RR investigators, Xu Wang, Caroline Watson, Joshua S. Sharp, James H. Prestegard, has joined with Tracy M. Handel from UCSD, to pursue a driving biomedical project directed at understanding how glycosaminoglycans (GAGs) help regulate immune cell migration and chemokine signaling. The chemokine, CCL5 or RANTES, aids recruitment and induces endothelial transmigration of …

Resource investigators actively disseminate our technologies through presentations, publication in journals and news articles. Representation at International Meetings Numerous presentations at International Symposium on Glycoconjugates, Proteoglycan and Glycobiology Gordon conferences and the Society for Glycobiology meetings. More than 50 presentations annually credit resource technologies, and investigators have organized, Chaired, or Co-chaired of over 10 glycoscience …