Although hundreds of heparan sulfate binding proteins have been identified, and implicated in a myriad of physiological and pathological processes, very little information is known about ligand requirements for binding and mediating biological activities by these proteins. This difficulty results from a lack of technology...
A team of Research Resource investigators, including members of the labs of Joshua Sharp and Kelley Moremen, have worked together to help determine the role of glycosaminoglycans (GAGs) in nervous system development. GAGs bridge a complex of two proteins, Robo1 and Slit2, to help...
Link modules found in a number of cell surface proteins play important roles in stabilizing the extracellular matrix (ECM) by interacting with glycosaminoglycans (GAGs). However the specificity and geometry of these interactions is poorly defined. Driven by a collaborative project with the Tony...
Glycosaminoglycan builder provides a point-and-click interface to build custom structures for all classes of glycosaminoglycans (GAGs). The web-tool is a simple interface where the user first chooses the desired class of GAG molecule and then enters the primary sequence by clicking through the set of...
A collaboration between UGA scientist Jonathan Amster, Robert Linhardt, at Rensselaer Polytechnic Institute, and Toshihiko Toida, at Chiba University, has led to the first ever sequencing of full length glycans from a naturally occurring proteoglycan. Although much of the structure of the protein portion of...
Co-receptor function of heparan sulfate in Slit3-Robo4 signaling: Heparan sulfate, carried by the heparan sulfate proteoglycans, such as syndecan, binds Slit3 on wildtype endothelial cell surface to form the heparan sulfate-Slit3-Robo4 ternary complexes facilitating Slit3-Robo4 signaling. Genetic ablation of N-deacetylase-N-sulfotransferase-1 (Ndst1), an dual function...
Through a collaboration between synthetic chemists investigating chemical synthesis of defined heparan sulfate oligosaccharides (Chengli Zong, Andre Venot, and Geert-Jan Boons), analytical chemists developing a derivatization, separation, and MS/MS methods for separating and sequencing complex mixtures of heparan sulfate oligosaccharides (Rongrong Huang, Dandan Zhou, and...
A platform for recombinant expression of mammalian glycoproteins has been developed in the Moremen lab for biochemical and structural studies on glycosylation enzymes and glycan binding proteins. Successful production of mammalian glycosylation enzymes in the Moremen lab led to the expanded development of a large-scale...
A team of RR investigators, Xu Wang, Caroline Watson, Joshua S. Sharp, James H. Prestegard, has joined with Tracy M. Handel from UCSD, to pursue a driving biomedical project directed at understanding how glycosaminoglycans (GAGs) help regulate immune cell migration and chemokine signaling. The chemokine,...
[vc_row css_animation="" row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern"][vc_column][vc_column_text]Resource investigators actively disseminate our technologies through presentations, publication in journals and news articles. Representation at International Meetings Numerous presentations at International Symposium on Glycoconjugates, Proteoglycan and Glycobiology Gordon conferences and the Society for Glycobiology meetings. More than 50 presentations...